primary antibodies against pink1  (Abmart Inc)

Journal: Redox Biology

Article Title: The UCP2/PINK1/LC3b-mediated mitophagy is involved in the protection of NRG1 against myocardial ischemia/reperfusion injury

doi: 10.1016/j.redox.2025.103511

Figure Lengend Snippet: NRG-1 postconditioning attenuated myocardial I/R injury and inhibited myocardial inflammatory responses. ( A ) Representative appearances the I/R myocardium with Evans blue and TTC staining. ( B ) Quantitative analysis of infarct area ( n = 5). ( C, D ) Serum cTnI and CK-MB concentrations ( n = 5). ( E ) Representative pathological appearances of the I/R myocardium with H & E staining (magnification, × 20; inset, × 40; scale bar: 100/50 μm). ( F ) Representative western blots for interleukin-1 β (IL-1β, 17 kDa) and Tubulin (49 kDa, loading control) in the I/R myocardium. ( G ) Quantitative analysis of western blotting data for IL-1β in the I/R myocardium ( n = 5). The Unpaired Student's t -test for between-group comparisons of data and one-way ANOVA followed with post hoc test (Bonferroni test) for multi-group comparisons. Data represent means ± SD. ★ P < 0.05, # P < 0.001. The unprocessed and extra duplicate images of western blots are provided in supplementry file .

Article Snippet: After washing with 0.05 % Tween-20 in the Tris-buffered saline (TBST), the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5 % BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology, Wuhan, China), UCP2 (Santa Cruz Biotechnology), PINK1 (Cell Signaling Technology) and α-tubulin (Santa Cruz Biotechnology) overnight at 4 °C.

Techniques: Staining, Western Blot, Control

Journal: Redox Biology

Article Title: The UCP2/PINK1/LC3b-mediated mitophagy is involved in the protection of NRG1 against myocardial ischemia/reperfusion injury

doi: 10.1016/j.redox.2025.103511

Figure Lengend Snippet: The cardioprotection of NRG-1 postconditioning was associated with mitophagy activation . ( A, B ) The PCA map of genes and volcano map of DEGs in the I/R myocardium from the IRI and NRG-1 groups ( n = 4, P < 0.05). ( C ) The KEGG analysis of DEGs ( n = 4, P < 0.05). ( D ) The GSEA analysis of mitophagy related genes with DEGs ( n = 4, p value is indicated on the figure). ( E ) The heat map of mitophagy related genes in the I/R myocardium from the IRI and NRG-1 groups ( n = 4).

Article Snippet: After washing with 0.05 % Tween-20 in the Tris-buffered saline (TBST), the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5 % BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology, Wuhan, China), UCP2 (Santa Cruz Biotechnology), PINK1 (Cell Signaling Technology) and α-tubulin (Santa Cruz Biotechnology) overnight at 4 °C.

Techniques: Activation Assay

Journal: Redox Biology

Article Title: The UCP2/PINK1/LC3b-mediated mitophagy is involved in the protection of NRG1 against myocardial ischemia/reperfusion injury

doi: 10.1016/j.redox.2025.103511

Figure Lengend Snippet: Inhibiting mitophagy eliminated the cardioprotective effects of NRG-1 postconditioning . ( A ) Representative TEM images of mitochondria in the I/R myocardium from different groups (magnification, × 5000; inset, × 10000; scale bar: 2/1 μm). ( B ) Representative western blots for LC3B (15 kDa), PINK1 (63 kDa) and Tubulin (49 kDa, loading control) in the I/R myocardium. ( C , D ) Quantification of western blotting data for LC3B and PINK1 in the I/R myocardium ( n = 5). ( E ) Representative confocal images of LC3B merged with DAPI in the I/R myocardium (magnification, × 20; cale bar: 100 μm). ( F ) Representative pathological appearances of the I/R myocardium with H&E staining (magnification, × 20; inset, × 40; scale bar: 100/50 μm). ( G , H ) Serum concentrations of cTnI and CK-MB ( n = 5). The Unpaired Student's t -test for between-group comparisons of data and one-way ANOVA followed with post hoc test (Bonferroni test) for multi-group comparisons. Data represent means ± SD. ★ P < 0.05, # P < 0.001, § P < 0.0001. The unprocessed and extra duplicate images of western blots are provided in supplementry file .

Article Snippet: After washing with 0.05 % Tween-20 in the Tris-buffered saline (TBST), the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5 % BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology, Wuhan, China), UCP2 (Santa Cruz Biotechnology), PINK1 (Cell Signaling Technology) and α-tubulin (Santa Cruz Biotechnology) overnight at 4 °C.

Techniques: Western Blot, Control, Staining

Journal: Redox Biology

Article Title: The UCP2/PINK1/LC3b-mediated mitophagy is involved in the protection of NRG1 against myocardial ischemia/reperfusion injury

doi: 10.1016/j.redox.2025.103511

Figure Lengend Snippet: The regulatory effect of NRG-1 on mitophagy was associated with UCP2 . ( A ) The Venn Diagram of the shared genes from the DEGs and PCGs associated with mitophagy ( n = 4). ( B – E ) The correlation analysis between shared genes and mitophagy ( n = 4, p value is indicated on the figure). ( F )The PPI analysis between UCP2 and mitophagy-related proteins (the connection between the two indicates evidence of interaction, the thickness of the connection correlates with the strength of the evidence).

Article Snippet: After washing with 0.05 % Tween-20 in the Tris-buffered saline (TBST), the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5 % BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology, Wuhan, China), UCP2 (Santa Cruz Biotechnology), PINK1 (Cell Signaling Technology) and α-tubulin (Santa Cruz Biotechnology) overnight at 4 °C.

Techniques:

Journal: Redox Biology

Article Title: The UCP2/PINK1/LC3b-mediated mitophagy is involved in the protection of NRG1 against myocardial ischemia/reperfusion injury

doi: 10.1016/j.redox.2025.103511

Figure Lengend Snippet: The UCP2 was located at the upstream of myocardial mitophagy to mediate the regulatory role of NRG-1 postconditioning . ( A ) Representative western blots for UCP2 (33 kDa), LC3B (15 kDa), PINK1 (63 kDa) and Tubulin (49 kDa, loading control) in the I/R myocardium. ( B – D ) The quantitative analyses of western blotting data for UCP2, LC3B and PINK1 in the I/R myocardium ( n = 5). ( E ) Representative confocal images of UCP2 merged with DAPI in the I/R myocardium (magnification, × 20; cale bar: 100 μm). The Unpaired Student's t -test for between-group comparisons of data and one-way ANOVA followed with post hoc test (Bonferroni test) for multi-group comparisons. Data represent means ± SD. ★ P < 0.05, # P < 0.001. The unprocessed and extra duplicate images of western blots are provided in supplementry file .

Article Snippet: After washing with 0.05 % Tween-20 in the Tris-buffered saline (TBST), the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5 % BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology, Wuhan, China), UCP2 (Santa Cruz Biotechnology), PINK1 (Cell Signaling Technology) and α-tubulin (Santa Cruz Biotechnology) overnight at 4 °C.

Techniques: Western Blot, Control

Journal: Redox Biology

Article Title: The UCP2/PINK1/LC3b-mediated mitophagy is involved in the protection of NRG1 against myocardial ischemia/reperfusion injury

doi: 10.1016/j.redox.2025.103511

Figure Lengend Snippet: NRG-1 postconditioning induced mitophagy in the cardiomyocytes subjected to H/R procedures . ( A ) Representative western blots for UCP2 (33 kDa), PINK1 (63 kDa) and Tubulin (49 kDa, loading control) in the H/R cardiomyocytes. ( B , C ) The quantitative analyses of western blotting data for UCP2 and PINK1 in the H/R cardiomyocytes ( n = 5). ( D ) Representative confocal images of JC-1 staining merged with DAPI in the H/R cardiomyocytes (magnification, × 60; cale bar: 20 μm). The Unpaired Student's t -test for between-group comparisons of data and one-way ANOVA followed with post hoc test (Bonferroni test) for multi-group comparisons. Data represent means ± SD. ★ P < 0.05, # P < 0.001. The unprocessed and extra duplicate images of western blots are provided in supplementry file .

Article Snippet: After washing with 0.05 % Tween-20 in the Tris-buffered saline (TBST), the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5 % BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology, Wuhan, China), UCP2 (Santa Cruz Biotechnology), PINK1 (Cell Signaling Technology) and α-tubulin (Santa Cruz Biotechnology) overnight at 4 °C.

Techniques: Western Blot, Control, Staining

Journal: Redox Biology

Article Title: The UCP2/PINK1/LC3b-mediated mitophagy is involved in the protection of NRG1 against myocardial ischemia/reperfusion injury

doi: 10.1016/j.redox.2025.103511

Figure Lengend Snippet: NRG-1 promoted mitophagy in the H/R cardiomyocytes by upregulating the UCP2-PINK1-LC3B signaling pathway. ( A ) Representative western blots for UCP2 (33 kDa), PINK1 (63 kDa), LC3B (15 kDa) and Tubulin (49 kDa, loading control) in the H/R cardiomyocytes with or without UCP2 siRNA challenge. ( B – D ) The quantitative analyses of western blotting data for UCP2, PINK1 and LC3B in the H/R cardiomyocytes ( n = 5). ( E ) Representative confocal images of colocalization of LC3B and mitochondria and merged with DAPI in the H/R cardiomyocytes (yellow fluorescence indicates the occurrence of colocalization; magnification, × 60; cale bar: 20 μm). ( F ) The LDH activity in the supernatants of the cultured cardiomyocytes ( n = 5). The Unpaired Student's t -test for between-group comparisons of data and one-way ANOVA followed with post hoc test (Bonferroni test) for multi-group comparisons. Data represent means ± SD. ★ P < 0.05, # P < 0.001, § P < 0.0001. The unprocessed and extra duplicate images of western blots are provided in supplementry file .

Article Snippet: After washing with 0.05 % Tween-20 in the Tris-buffered saline (TBST), the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5 % BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology, Wuhan, China), UCP2 (Santa Cruz Biotechnology), PINK1 (Cell Signaling Technology) and α-tubulin (Santa Cruz Biotechnology) overnight at 4 °C.

Techniques: Western Blot, Control, Fluorescence, Activity Assay, Cell Culture

Journal: Redox Biology

Article Title: The UCP2/PINK1/LC3b-mediated mitophagy is involved in the protection of NRG1 against myocardial ischemia/reperfusion injury

doi: 10.1016/j.redox.2025.103511

Figure Lengend Snippet: NRG-1 postconditioning attenuated myocardial I/R injury via the UCP2-PINK1-LC3B signaling pathway. ( A ) Representative western blots for UCP2 (33 kDa), LC3B (15 kDa), PINK1 (63 kDa) and Tubulin (49 kDa, loading control) in the I/R myocardium. ( B – D ) The quantitative analyses of western blotting data for UCP2, LC3B and PINK1 in the I/R myocardium ( n = 5). ( E ) Representative confocal images of UCP2 merged with DAPI in the I/R myocardium (magnification, × 20; cale bar: 100 μm). ( F ) Representative pathological appearances of the I/R myocardium with H & E staining (magnification, × 20; inset, × 40; scale bar: 100/50 μm). ( G , H ) Serum cTnI and CK-MB concentrations ( n = 5). The Unpaired Student's t -test for between-group comparisons of data and one-way ANOVA followed with post hoc test (Bonferroni test) for multi-group comparisons. Data represent means ± SD. ★ P < 0.05; # P < 0.001; § P < 0.0001. The unprocessed and extra duplicate images of western blots are provided in supplementry file .

Article Snippet: After washing with 0.05 % Tween-20 in the Tris-buffered saline (TBST), the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5 % BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology, Wuhan, China), UCP2 (Santa Cruz Biotechnology), PINK1 (Cell Signaling Technology) and α-tubulin (Santa Cruz Biotechnology) overnight at 4 °C.

Techniques: Western Blot, Control, Staining

Journal: Redox Biology

Article Title: The UCP2/PINK1/LC3b-mediated mitophagy is involved in the protection of NRG1 against myocardial ischemia/reperfusion injury

doi: 10.1016/j.redox.2025.103511

Figure Lengend Snippet: Schematic illustration exhibiting the molecular mechanism that NRG-1 postconditioning activates mitophagy to provide a protection against myocardial I/R injury . The NRG-1 treatment upregulates the UCP2 expression in the mitochondria of cardiomyocytes. The UCP2 is able to reduce the mitochondrial membrane potential (MMP), which subsequently leads to the accumulation of the PINK1 complex on the outer membrane of mitochondria. This process induces the lysosomal surface recognizer LC3B to bind to the complex, ultimately resulting in the activation of mitophagy.

Article Snippet: After washing with 0.05 % Tween-20 in the Tris-buffered saline (TBST), the membranes were incubated at a room temperature condition for 2 h with a blocking solution containing 5 % BSA and then incubated with specific primary antibody against IL-1β (Cell Signaling Technology, Danvers, Massachusetts, USA), LC3B (ABclonal Technology, Wuhan, China), UCP2 (Santa Cruz Biotechnology), PINK1 (Cell Signaling Technology) and α-tubulin (Santa Cruz Biotechnology) overnight at 4 °C.

Techniques: Expressing, Membrane, Activation Assay